Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)

Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nuc...

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Main Author:	Mohd Mohidin, Taznim Begam
Format:	Thesis
Language:	English
Published:	2006
Subjects:	Nipah virus - Research
Online Access:	http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf
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id	my-upm-ir.177
record_format	uketd_dc
spelling	my-upm-ir.1772021-08-18T08:25:50Z Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) 2006-02 Mohd Mohidin, Taznim Begam Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication. Nipah virus - Research 2006-02 Thesis http://psasir.upm.edu.my/id/eprint/177/ http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf text en public masters Universiti Putra Malaysia Nipah virus - Research Faculty of Biotechnology and Biomolecular Sciences
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Nipah virus - Research
spellingShingle	Nipah virus - Research Mohd Mohidin, Taznim Begam Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
description	Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication.
format	Thesis
qualification_level	Master's degree
author	Mohd Mohidin, Taznim Begam
author_facet	Mohd Mohidin, Taznim Begam
author_sort	Mohd Mohidin, Taznim Begam
title	Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
title_short	Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
title_full	Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
title_fullStr	Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
title_full_unstemmed	Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
title_sort	specific interactions betweeen nipah virus nucleocapsid (n) protein and phospho-(p) protein using the yeast two-hybrid system (vector page tiada)
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf
_version_	1747810167094771712

Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)

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