Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nuc...
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my-upm-ir.1772021-08-18T08:25:50Z Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) 2006-02 Mohd Mohidin, Taznim Begam Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication. Nipah virus - Research 2006-02 Thesis http://psasir.upm.edu.my/id/eprint/177/ http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf text en public masters Universiti Putra Malaysia Nipah virus - Research Faculty of Biotechnology and Biomolecular Sciences |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English |
| topic |
Nipah virus - Research |
| spellingShingle |
Nipah virus - Research Mohd Mohidin, Taznim Begam Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| description |
Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family
Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other
known paramyxoviruses. In this study, the region(s) involved in the interaction between
this exceptionally large P protein with its nucleocapsid (N) protein was investigated in
vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s)
of both the N and P proteins involved in N-P and P-N interactions. Mapping of the
domains of N protein involved in its interaction with the P protein revealed that the Cterminal
30 amino acids (423-452 residues) are crucial for N-P interaction. However,
mapping of the domains of P protein involved in the P-N association demonstrated that
both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62
amino acids (1-62 residues) simultaneously play a major role. Comparison of these
findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome
replication. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Mohd Mohidin, Taznim Begam |
| author_facet |
Mohd Mohidin, Taznim Begam |
| author_sort |
Mohd Mohidin, Taznim Begam |
| title |
Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_short |
Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_full |
Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_fullStr |
Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_full_unstemmed |
Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_sort |
specific interactions betweeen nipah virus nucleocapsid (n) protein and phospho-(p) protein using the yeast two-hybrid system (vector page tiada) |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty of Biotechnology and Biomolecular Sciences |
| publishDate |
2006 |
| url |
http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf |
| _version_ |
1747810167094771712 |