Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects

Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects

This study was carried out to determine nutritional composition, antioxidant properties of red pitaya (Hylocereus polyrhizus) powder (RPP) and to evaluate the effects of RPP consumption on blood glucose level and lipid profiles in streptozotocin-induced diabetic rats and pre-diabetic human subjects....

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Main Author: Akhiruddin, Mohd Al-Saufreen
Format: Thesis
Language:English
Published: 2013
Subjects:
Diabetes Mellitus
Online Access:http://psasir.upm.edu.my/id/eprint/38648/1/FPSK%28m%29%202013%2038%20IR.pdf
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spelling my-upm-ir.386482016-02-26T08:47:43Z Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects 2013-05 Akhiruddin, Mohd Al-Saufreen This study was carried out to determine nutritional composition, antioxidant properties of red pitaya (Hylocereus polyrhizus) powder (RPP) and to evaluate the effects of RPP consumption on blood glucose level and lipid profiles in streptozotocin-induced diabetic rats and pre-diabetic human subjects. The study consisted of three phases. In phase one, the proximate analyses of RPP were determined according to the methods of Association of Official Analytical Chemists (AOAC) international. Meanwhile, ascorbic acid assessment was carried out according to the modified method using high performance liquid chromatography (HPLC) assay. The RPP antioxidant power was determined using FRAP assay. The proximate composition of RPP were moisture (5.27 %), ash (1.22 g), carbohydrate (26.6 g), protein (1.59 g), fat (0.014 g), soluble fiber (12.05g) and insoluble fiber (5.65 g). The vitamin C content was 32.14 mg/100 g. The FRAP assay value of RPP was 31.36 ± 3.45 μM/100g or 8.72 ± 0.16 mg/100g. In phase two, study on hypoglycemic effects of RPP on plasma glucose level and lipid profiles in diabetic induced rats was conducted. This study used forty eight male Sprague-Dawley rats weight between 250 g-300 g. All the rats were divided equally into six groups. The groups consisted of control groups (NC & DC) and treatment groups (NPP, DPP, DPPM & DM). After four weeks of treatment, all treatment groups had showed significantly reduced (p<0.05) in the plasma glucose level. The highest percentage reduction in glucose level was observed in DPPM group with 69.97 %. For plasma total cholesterol (TC) level, the highest percentage reduction was observed in NPP group with 62.39 %. In phase three, community trials were conducted among pre-diabetic human subjects at Felda Mempaga Bentong, Pahang. Total thirty six subjects were randomly divided into two groups which were pre-diabetic treatment groups consisted of PT3 (consumed 60 g RPP/day), PT4 (consumed 80 g RPP/day), and PT5 (consumed 100 g RPP/day) and pre-diabetic control groups which were not consumed RPP. For plasma glucose level, among treatment groups, PT5 had showed the highest percentage of reduction (22.90 %) with significant difference (p<0.05) in the mean plasma blood glucose level after 4 weeks of treatment. For plasma lipid profiles, showed that group PT5 had the highest percentage of reduction with significant difference (p<0.05) in TC (26.44 %), TG (20.54 %), and LDL-C (49.55%) levels followed by PT4 and PT3 groups after 4 weeks of treatment. For HDL-C level, PT5 group also showed the highest percentage of increment 63.8 %. The results revealed that all dosages of RPP drinks showed positive effects in increasing HDL-C and TAS levels, in the same time lowering glucose, TC, TG and LDL-C levels in pre-diabetic subjects. Diabetes Mellitus 2013-05 Thesis http://psasir.upm.edu.my/id/eprint/38648/ http://psasir.upm.edu.my/id/eprint/38648/1/FPSK%28m%29%202013%2038%20IR.pdf application/pdf en public masters Universiti Putra Malaysia Diabetes Mellitus
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
topic Diabetes Mellitus
spellingShingle Diabetes Mellitus


Akhiruddin, Mohd Al-Saufreen
Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
description This study was carried out to determine nutritional composition, antioxidant properties of red pitaya (Hylocereus polyrhizus) powder (RPP) and to evaluate the effects of RPP consumption on blood glucose level and lipid profiles in streptozotocin-induced diabetic rats and pre-diabetic human subjects. The study consisted of three phases. In phase one, the proximate analyses of RPP were determined according to the methods of Association of Official Analytical Chemists (AOAC) international. Meanwhile, ascorbic acid assessment was carried out according to the modified method using high performance liquid chromatography (HPLC) assay. The RPP antioxidant power was determined using FRAP assay. The proximate composition of RPP were moisture (5.27 %), ash (1.22 g), carbohydrate (26.6 g), protein (1.59 g), fat (0.014 g), soluble fiber (12.05g) and insoluble fiber (5.65 g). The vitamin C content was 32.14 mg/100 g. The FRAP assay value of RPP was 31.36 ± 3.45 μM/100g or 8.72 ± 0.16 mg/100g. In phase two, study on hypoglycemic effects of RPP on plasma glucose level and lipid profiles in diabetic induced rats was conducted. This study used forty eight male Sprague-Dawley rats weight between 250 g-300 g. All the rats were divided equally into six groups. The groups consisted of control groups (NC & DC) and treatment groups (NPP, DPP, DPPM & DM). After four weeks of treatment, all treatment groups had showed significantly reduced (p<0.05) in the plasma glucose level. The highest percentage reduction in glucose level was observed in DPPM group with 69.97 %. For plasma total cholesterol (TC) level, the highest percentage reduction was observed in NPP group with 62.39 %. In phase three, community trials were conducted among pre-diabetic human subjects at Felda Mempaga Bentong, Pahang. Total thirty six subjects were randomly divided into two groups which were pre-diabetic treatment groups consisted of PT3 (consumed 60 g RPP/day), PT4 (consumed 80 g RPP/day), and PT5 (consumed 100 g RPP/day) and pre-diabetic control groups which were not consumed RPP. For plasma glucose level, among treatment groups, PT5 had showed the highest percentage of reduction (22.90 %) with significant difference (p<0.05) in the mean plasma blood glucose level after 4 weeks of treatment. For plasma lipid profiles, showed that group PT5 had the highest percentage of reduction with significant difference (p<0.05) in TC (26.44 %), TG (20.54 %), and LDL-C (49.55%) levels followed by PT4 and PT3 groups after 4 weeks of treatment. For HDL-C level, PT5 group also showed the highest percentage of increment 63.8 %. The results revealed that all dosages of RPP drinks showed positive effects in increasing HDL-C and TAS levels, in the same time lowering glucose, TC, TG and LDL-C levels in pre-diabetic subjects.
format Thesis
qualification_level Master's degree
author Akhiruddin, Mohd Al-Saufreen
author_facet Akhiruddin, Mohd Al-Saufreen
author_sort Akhiruddin, Mohd Al-Saufreen
title Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
title_short Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
title_full Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
title_fullStr Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
title_full_unstemmed Nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
title_sort nutritional composition, antioxidant properties of hylocereus polyrhizus powder and their effects on plasma glucose level and lipid profiles in diabetic rats and pre-diabetic subjects
granting_institution Universiti Putra Malaysia
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/38648/1/FPSK%28m%29%202013%2038%20IR.pdf
_version_ 1747811743243960320

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