Kinetics, crystallization, and structural elucidation of thermostable D311E T1 lipase variant from Geobacillus zalihae

T1 lipase from Geobacillus zalihae is a thermophilic enzyme with an optimum temperature of 70 oC at pH 9 and stable up to 65 oC. By introducing an additional ion pair, T1 lipase was believed to be stable at high temperature. Thus, further study of D311E lipase variant are required to determine the e...

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Bibliographic Details
Main Author: Ruslan, Rudzanna
Format: Thesis
Published: 2013
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