Stability of Algerian black seed (Nigella sativa L.) in relation to antimicrobial activity under different storage conditions
The present study is an effort to determine the stability of the main volatile constituents of Nigella sativa seeds stored under several conditions. Nine storage conditions which were set based on the ecological abiotic effects of air, humidity, heat and light, with six replicates each were pr...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/67987/1/IB%202016%2020%20IR.pdf |
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| Summary: | The present study is an effort to determine the stability of the main volatile constituents
of Nigella sativa seeds stored under several conditions. Nine storage conditions which
were set based on the ecological abiotic effects of air, humidity, heat and light, with six
replicates each were prepared and analyzed with Headspace (HS)-Gas
Chromatography-Mass Spectrometry (GCMS) for three time points of initial (1st day –
0), 14th (1) and 28th (2) day of storage. A targeted multivariate analysis of PCA revealed
that the stability of the whole seeds main volatile constituents were better than those of
the ground seeds in relation to the selected ecofactors. The findings showed that the air,
humidity, heat and light are suggested to be in reverse relationship with the stability of
N. sativa seeds. Based on the study, the condition which showed minimal changes
towards the environmental effects is that whole seeds stored in a transparent vial with
nitrogen gas flush. The disc-diffusion test antimicrobial activity of the different stored
conditions of N. sativa samples were conducted on several human pathogens wherein
the whole seed stored in limited air content (condition B and C) and exposed to light
gave more promising results. However, the minimum inhibition concentration (MIC)
and minimum bactericidal concentration (MBC) results showed no significant
difference among all conditions after 28 days storage. The antimicrobial metabolites
were identified through correlation of antimicrobial activities (disc-diffusion test) with
N. sativa whole seeds of different storage conditions by supervised multivariate data
analysis (MVA) of Orthogonal Partial Least Squares (OPLS), which suggested that 7
significant variables responsible as chemical markers. Furthermore, the predictability
of antimicrobial activity based on the whole seed model was done on 10 new
observations of known GC-MS data whereby only fresh whole seed (MEOH) sample
was positively predicted. Based on the present findings, N. sativa seeds need suitable
storage parameters to preserve the volatiles for less quality deprivation. |
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