Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter

Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter

Thirty five litter samples of village chickens, 35 broilers and 32 samples of raw milk were examined for the presence of E. coli. All samples were positive for E. coli. Three hundred and five isolates of E. coli were isolated from litter samples of village chickens (105 isolates), broilers (105 i...

Full description

Saved in:
Bibliographic Details
Main Author: Lihan, Samuel
Format: Thesis
Language:English
English
Published: 1999
Subjects:
Escherichia coli infections in animals.
Online Access:http://psasir.upm.edu.my/id/eprint/8405/1/FSMB_1999_8_A.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-upm-ir.8405
record_format uketd_dc
spelling my-upm-ir.84052012-07-03T02:53:42Z Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter 1999 Lihan, Samuel Thirty five litter samples of village chickens, 35 broilers and 32 samples of raw milk were examined for the presence of E. coli. All samples were positive for E. coli. Three hundred and five isolates of E. coli were isolated from litter samples of village chickens (105 isolates), broilers (105 isolates) and raw milk samples (95 isolates). All the isolates were examined for antibiotic resistance, plasmid profiles and polymorphism using random amplification of polymorphic DNA (RAPD) analysis. Isolates isolated from litter of village chickens and broilers had a multiple antibiotic resistance (MAR) index of 0.31 to 0.75 and 0.44 to 0.69, respectively. Isolates isolated from raw milk had a MAR index of 0.31 to 0.88. High MAR index suggests that all the isolates originated from high risk sources. The E. coli isolates isolated from village chickens, broilers and raw milk samples were grouped into 34,30 and 28 distinct antibiotypes, respectively. Eighty (76.2%) and 99 (94.3%) isolates were found to harbour plasmids ranging in size from 1.2 to 64 MDa and 1.2 to 80 MDa among isolates isolated from village chickens and broilers, respectively. Isolates isolated from raw milk harboured plasmids ranging in size from 1.4 to 68 MDa. Based on their plasmid profiles, the E. coli isolates isolated from village chickens, broilers and raw milk were grouped into 28, 57 and 5 plasmid patterns, respectively. Three l0-mer oligonucleotides primers (Gen1-50-02, Gen1-50-09 and Gen1-50-10) were used to amplify genomic DNA. The profiles observed after electrophoretic separation for the three primers when combined together were able to distinguish the E. coli isolates from village chickens, broilers and raw milk into 92, 96 and 50 RAPD patterns, respectively. The large number of subgroups within these isolates indicates that there is a high degree of diversity within E. coli isolates, isolated from village chickens, broilers and raw milk samples. Isolates, isolated from village chickens, broilers and raw milk are genotypically diverse as shown by RAPD pattern, suggesting that different strains have been brought into the geographic region and strains already present have continued to evolve. These results suggest that RAPD-PCR assay is more discriminating than plasmid profiling and antibiotyping, and could be a valuable tool for epidemiological studies. Escherichia coli infections in animals. 1999 Thesis http://psasir.upm.edu.my/id/eprint/8405/ http://psasir.upm.edu.my/id/eprint/8405/1/FSMB_1999_8_A.pdf application/pdf en public masters Universiti Putra Malaysia Escherichia coli infections in animals. Food Science and Technology English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Escherichia coli infections in animals.
spellingShingle Escherichia coli infections in animals.


Lihan, Samuel
Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
description Thirty five litter samples of village chickens, 35 broilers and 32 samples of raw milk were examined for the presence of E. coli. All samples were positive for E. coli. Three hundred and five isolates of E. coli were isolated from litter samples of village chickens (105 isolates), broilers (105 isolates) and raw milk samples (95 isolates). All the isolates were examined for antibiotic resistance, plasmid profiles and polymorphism using random amplification of polymorphic DNA (RAPD) analysis. Isolates isolated from litter of village chickens and broilers had a multiple antibiotic resistance (MAR) index of 0.31 to 0.75 and 0.44 to 0.69, respectively. Isolates isolated from raw milk had a MAR index of 0.31 to 0.88. High MAR index suggests that all the isolates originated from high risk sources. The E. coli isolates isolated from village chickens, broilers and raw milk samples were grouped into 34,30 and 28 distinct antibiotypes, respectively. Eighty (76.2%) and 99 (94.3%) isolates were found to harbour plasmids ranging in size from 1.2 to 64 MDa and 1.2 to 80 MDa among isolates isolated from village chickens and broilers, respectively. Isolates isolated from raw milk harboured plasmids ranging in size from 1.4 to 68 MDa. Based on their plasmid profiles, the E. coli isolates isolated from village chickens, broilers and raw milk were grouped into 28, 57 and 5 plasmid patterns, respectively. Three l0-mer oligonucleotides primers (Gen1-50-02, Gen1-50-09 and Gen1-50-10) were used to amplify genomic DNA. The profiles observed after electrophoretic separation for the three primers when combined together were able to distinguish the E. coli isolates from village chickens, broilers and raw milk into 92, 96 and 50 RAPD patterns, respectively. The large number of subgroups within these isolates indicates that there is a high degree of diversity within E. coli isolates, isolated from village chickens, broilers and raw milk samples. Isolates, isolated from village chickens, broilers and raw milk are genotypically diverse as shown by RAPD pattern, suggesting that different strains have been brought into the geographic region and strains already present have continued to evolve. These results suggest that RAPD-PCR assay is more discriminating than plasmid profiling and antibiotyping, and could be a valuable tool for epidemiological studies.
format Thesis
qualification_level Master's degree
author Lihan, Samuel
author_facet Lihan, Samuel
author_sort Lihan, Samuel
title Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
title_short Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
title_full Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
title_fullStr Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
title_full_unstemmed Molecular Characterisation of Escherichia Coli Isolated from Raw Milk and Village Chicken and Broiler Litter
title_sort molecular characterisation of escherichia coli isolated from raw milk and village chicken and broiler litter
granting_institution Universiti Putra Malaysia
granting_department Food Science and Technology
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/8405/1/FSMB_1999_8_A.pdf
_version_ 1747810799102984192

Similar Items