Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat

Thirty isolates of Listeria monocytogenes (12) and Listeria innocua (18) were isolated from poultry meat. All isolates were confirmed by Microbact (Medvet, Australia) identification kits. All the isolates were subjected to chromosomal and plasmid DNA screening and antibiotic resistance test. Base...

Full description

Saved in:

Bibliographic Details
Main Author:	Bilung, Lesley Maurice
Format:	Thesis
Language:	English English
Published:	2002
Subjects:	Listeria monocytogenes. Listeria. Listeriosis in animals.
Online Access:	http://psasir.upm.edu.my/id/eprint/8478/1/FSMB_2002_15_IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.8478
record_format	uketd_dc
spelling	my-upm-ir.84782024-01-26T07:31:51Z Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat 2002-04 Bilung, Lesley Maurice Thirty isolates of Listeria monocytogenes (12) and Listeria innocua (18) were isolated from poultry meat. All isolates were confirmed by Microbact (Medvet, Australia) identification kits. All the isolates were subjected to chromosomal and plasmid DNA screening and antibiotic resistance test. Based on the antibiotic resistance profiles, Listeria monocytogenes and Listeria innocua were differentiated into 10 and 9 profiles respectively. The antibiotyping procedure discriminated the Listeria monocytogenes and Listeria innocua into 10 and 3 different groups respectively. Most of the isolates were resistant to nalidixic acid (100%), c1indamycin (97%), spectinomycin (97%), cefuroxime (93%), cefriaxone (80%), cephalothin (73%), cefotaxime (67%), novobiocin (37%), chloramphenicol (27%), kanamycin (20%), rifampicin (20%), tobramycin (17%), norfloxacin (13%), netilmicin(10%) and imipenem (3%). The results of the plasmid profiles and antibiotyping show that there is no correlation between them. RAPD-PCR has been used to generate polymorphic genomic fingerprints to discriminate the Listeria isolates. Primer GEN15009 was chosen whereby it produced reproducible and typeable results in all isolates examined with the bands ranging from 0.25 to 3.0 kilobase pairs. From the dendrogram generated L. monocytogenes were separated from L. innocua and the strains in each species were differentiated as well. The data indicate that RAPD-PCR based approaches is a valid means of discriminating strain differences among isolates of L. monocytogenes and L. innocua and as an adjunct to differentiate among Listeria spp. Listeria monocytogenes. Listeria. Listeriosis in animals. 2002-04 Thesis http://psasir.upm.edu.my/id/eprint/8478/ http://psasir.upm.edu.my/id/eprint/8478/1/FSMB_2002_15_IR.pdf text en public masters Universiti Putra Malaysia Listeria monocytogenes. Listeria. Listeriosis in animals. Food Science and Technology Radu, Son English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Radu, Son
topic	Listeria monocytogenes. Listeria. Listeriosis in animals.
spellingShingle	Listeria monocytogenes. Listeria. Listeriosis in animals. Bilung, Lesley Maurice Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
description	Thirty isolates of Listeria monocytogenes (12) and Listeria innocua (18) were isolated from poultry meat. All isolates were confirmed by Microbact (Medvet, Australia) identification kits. All the isolates were subjected to chromosomal and plasmid DNA screening and antibiotic resistance test. Based on the antibiotic resistance profiles, Listeria monocytogenes and Listeria innocua were differentiated into 10 and 9 profiles respectively. The antibiotyping procedure discriminated the Listeria monocytogenes and Listeria innocua into 10 and 3 different groups respectively. Most of the isolates were resistant to nalidixic acid (100%), c1indamycin (97%), spectinomycin (97%), cefuroxime (93%), cefriaxone (80%), cephalothin (73%), cefotaxime (67%), novobiocin (37%), chloramphenicol (27%), kanamycin (20%), rifampicin (20%), tobramycin (17%), norfloxacin (13%), netilmicin(10%) and imipenem (3%). The results of the plasmid profiles and antibiotyping show that there is no correlation between them. RAPD-PCR has been used to generate polymorphic genomic fingerprints to discriminate the Listeria isolates. Primer GEN15009 was chosen whereby it produced reproducible and typeable results in all isolates examined with the bands ranging from 0.25 to 3.0 kilobase pairs. From the dendrogram generated L. monocytogenes were separated from L. innocua and the strains in each species were differentiated as well. The data indicate that RAPD-PCR based approaches is a valid means of discriminating strain differences among isolates of L. monocytogenes and L. innocua and as an adjunct to differentiate among Listeria spp.
format	Thesis
qualification_level	Master's degree
author	Bilung, Lesley Maurice
author_facet	Bilung, Lesley Maurice
author_sort	Bilung, Lesley Maurice
title	Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
title_short	Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
title_full	Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
title_fullStr	Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
title_full_unstemmed	Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat
title_sort	isolation and molecular characterisation of listeria monocytogenes and listeria innocua from poultry meat
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/8478/1/FSMB_2002_15_IR.pdf
_version_	1794018757281054720

Isolation and Molecular Characterisation of Listeria Monocytogenes and Listeria Innocua from Poultry Meat

Similar Items