The Use of Zinc Finger Protein Gene to Detect the Presence of Y Chromosome in the Cow

Total genomic DNA from 24 normal cows and a normal male cattle were isolated from leucocyte preparation. Polymerase Chain Reaction (PCR) amplification of these genomic DNA using the PI-5EZ and P2-3EZ primers for ZFX/ZFY gene resulted in a fragment of approximately 440 bp in size from both male an...

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Bibliographic Details
Main Author: Shamsuddin, Shaharum
Format: Thesis
Published: 1996
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