Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii

Cronobacter sakazakii is a foodborne pathogen that has been implicated to be the main cause of neonate’s infections from reconstituted powdered infant formula and powdered milk. Attention is being focused on production of physiologically active compounds derived from milk proteins with antimicrobial...

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Main Author:	Ibrahim Mustafa Elshaafi
Format:	Thesis
Language:	English
Subjects:	Goat milk Lactic acid bacteria
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spelling	my-usim-ddms-123662024-05-29T04:02:02Z Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii Ibrahim Mustafa Elshaafi Cronobacter sakazakii is a foodborne pathogen that has been implicated to be the main cause of neonate’s infections from reconstituted powdered infant formula and powdered milk. Attention is being focused on production of physiologically active compounds derived from milk proteins with antimicrobial property that are effective against the foodborne pathogens are increasing. This study was carried out to investigate the capabilities of 30 lactic acid bacteria (LAB) isolates obtained from different sources to hydrolyse goat’s milk casein (GMC) and produce peptides with antimicrobial activity that can inhibit the growth C. sakazakii and other pathogens. Eight isolates showed good proteolytic activity with clear zones ranging from 7 to 12 mm on skim milk-MRS agar. Initial screening of GMC fermented with these LAB isolates showed that three of them produced hydrolysates that showed antimicrobial activity against the target pathogens C. sakazakii ATCC 25944, C. sakazakii E4 and C. sakazakii O531G and other pathogens. The three LAB were identified as Lactobacillus casei CF2, Enterococcus faecium F.F.B, and Lactobacillus plantarum S by bio-chemical tests, API CHL50, and 16s DNA sequencing. The inhibitory activity of goat milk casein hydrolysate (GMCH) against multi antibiotic resistant pathogens were significantly (P<0.05) affected by LAB isolates, fermentation time, and concentration of GMCH. Killing of target pathogen was observed within 2 h at 0.7 % GMC (v/v). Amino acid profiles of the GMCH varied in the concentration and types of amino acids depending on the LAB used to hydrolyse the GMC. The main amino acid present in the three LAB-GMCH was lysine. The lysine to arginine ratio of GMCH using L. casei-CF2 was 100:0, E. faecium-F.F.B was 91.3: 4.54, L. plantarum-S was 87: 1.58. The ratio of hydrophilic to hydrophobic amino acid of the GMCH also varies with the organism used to generate the peptides. The amino acid profile of the GMCH consisted of hydrophilic amino acid: 69.69% for L. casei-CF2- GMCH, 86.95% for E. faecium-F.F.B-GMCH and 79.68% for L. plantarum-S-GMCH. The results of this study showed potential antimicrobial activity against C. sakazakii by peptides generated from goat milk casein by LAB. Universiti Sains Islam Malaysia 2015 Thesis en https://oarep.usim.edu.my/handle/123456789/12366 https://oarep.usim.edu.my/bitstreams/9a13b913-cfb0-4efe-aa84-4bf8e5ad1ec6/download 8a4605be74aa9ea9d79846c1fba20a33 Goat milk Lactic acid bacteria
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Goat milk Lactic acid bacteria
spellingShingle	Goat milk Lactic acid bacteria Ibrahim Mustafa Elshaafi Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
description	Cronobacter sakazakii is a foodborne pathogen that has been implicated to be the main cause of neonate’s infections from reconstituted powdered infant formula and powdered milk. Attention is being focused on production of physiologically active compounds derived from milk proteins with antimicrobial property that are effective against the foodborne pathogens are increasing. This study was carried out to investigate the capabilities of 30 lactic acid bacteria (LAB) isolates obtained from different sources to hydrolyse goat’s milk casein (GMC) and produce peptides with antimicrobial activity that can inhibit the growth C. sakazakii and other pathogens. Eight isolates showed good proteolytic activity with clear zones ranging from 7 to 12 mm on skim milk-MRS agar. Initial screening of GMC fermented with these LAB isolates showed that three of them produced hydrolysates that showed antimicrobial activity against the target pathogens C. sakazakii ATCC 25944, C. sakazakii E4 and C. sakazakii O531G and other pathogens. The three LAB were identified as Lactobacillus casei CF2, Enterococcus faecium F.F.B, and Lactobacillus plantarum S by bio-chemical tests, API CHL50, and 16s DNA sequencing. The inhibitory activity of goat milk casein hydrolysate (GMCH) against multi antibiotic resistant pathogens were significantly (P<0.05) affected by LAB isolates, fermentation time, and concentration of GMCH. Killing of target pathogen was observed within 2 h at 0.7 % GMC (v/v). Amino acid profiles of the GMCH varied in the concentration and types of amino acids depending on the LAB used to hydrolyse the GMC. The main amino acid present in the three LAB-GMCH was lysine. The lysine to arginine ratio of GMCH using L. casei-CF2 was 100:0, E. faecium-F.F.B was 91.3: 4.54, L. plantarum-S was 87: 1.58. The ratio of hydrophilic to hydrophobic amino acid of the GMCH also varies with the organism used to generate the peptides. The amino acid profile of the GMCH consisted of hydrophilic amino acid: 69.69% for L. casei-CF2- GMCH, 86.95% for E. faecium-F.F.B-GMCH and 79.68% for L. plantarum-S-GMCH. The results of this study showed potential antimicrobial activity against C. sakazakii by peptides generated from goat milk casein by LAB.
format	Thesis
author	Ibrahim Mustafa Elshaafi
author_facet	Ibrahim Mustafa Elshaafi
author_sort	Ibrahim Mustafa Elshaafi
title	Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
title_short	Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
title_full	Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
title_fullStr	Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
title_full_unstemmed	Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii
title_sort	antimicrobial activity of goat milk casein generated by lactic acid bacteria used against cronobacter sakazakii
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444911675375616

Antimicrobial activity of Goat Milk Casein generated by Lactic Acid Bacteria used against Cronobacter Sakazakii

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