Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity
Sea cucumber is one of the most commercialized seafood products in Asia, including Malaysia, and its consumption has recently increased with recognition of its health beneficial values. This marine invertebrate is surrounded by microbial communities that may provide defence against pathogens and,...
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| id |
my-usim-ddms-12451 |
|---|---|
| record_format |
uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
en_US |
| topic |
Sea cucumber Bacteria Pigment 16S rRNA gene Antibacterial activity |
| spellingShingle |
Sea cucumber Bacteria Pigment 16S rRNA gene Antibacterial activity Fatin Najihah Binti Muhammad Lutfi Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| description |
Sea cucumber is one of the most commercialized seafood products in Asia, including
Malaysia, and its consumption has recently increased with recognition of its health
beneficial values. This marine invertebrate is surrounded by microbial communities that
may provide defence against pathogens and, in turn, the symbiotic relationship could be
a source of bioactive compounds. Many research on marine invertebrate-associated
bacteria have been carried out, yet limited studies have documented on bacteria from
various body parts of sea cucumbers in Malaysia seawaters, specifically on pigmented
bacteria. In this study, eight sea cucumbers were collected from Pangkor Island, Perak,
and Manukan Island, Sabah, and the specimens were identified as two H. leucospilota,
three H. atra, and one each of H. edulis, H. hilla and B. vitiensis. A total of 115 bacterial
samples were isolated from various body parts of the sea cucumbers, surrounding
sediments and seawaters, and 58 bacterial strains were successfully identified using 16S
rRNA gene amplification, DNA sequencing and phylogenetic analysis. Bacteria from
the genera Vibrio and Bacillus are the most affiliated with sea cucumber from Pangkor
Island and Manukan Island, respectively. Other bacterial strains belong to the genera
Acinetobacteria, Staphylococcus, Pseudomonas, Paracoccus, Microbacterium,
Brevundinomas, Exiguobacterium and Stenotrophomonas. Phylogenetic analysis
revealed that isolates were assigned to three primary cluster consisting of
Proteobacteria, Firmicutes and Actinobacteria phylum. Bacterial isolates that showed
pigmentation were selected for UV-Vis spectrophotmetry analysis and antibacterial
screening using disk diffusion method. The absorbance spectra of crude methanolic
extract of Pseudomonas sp. PMCC1 suggested potential phenazine-based pigments,
while those from Staphylococcus sp. PMDP, Microbacterium sp. PMCT1 and
Acinetobacteria sp. PMDS1 resembling carotenoid pigments. However, more studies
are needed to confirm these pigments. Four pigmented bacterial strains exhibited
antibacterial activity against at least one pathogenic bacterium, with Pseudomonas sp.
PMCC1 showed inhibition to all five tested bacteria, ranging from 13 to 22 mm. This
study suggests that the bacteria isolated from sea cucumbers may have great potential
in the discovery of useful bioactive compounds. Therefore, further studies are needed
to explore this vast pool of bacterial isolates, especially the pigment-producing bacteria
and to determine their biotechnological potentiality. |
| format |
Thesis |
| author |
Fatin Najihah Binti Muhammad Lutfi |
| author_facet |
Fatin Najihah Binti Muhammad Lutfi |
| author_sort |
Fatin Najihah Binti Muhammad Lutfi |
| title |
Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| title_short |
Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| title_full |
Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| title_fullStr |
Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| title_full_unstemmed |
Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity |
| title_sort |
identification and assessment of pigmented bacterial isolates from sea cucumbers for antibacterial activity |
| granting_institution |
Universiti Sains Islam Malaysia |
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1812444869354848256 |
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my-usim-ddms-124512024-05-29T20:19:10Z Identification And Assessment Of Pigmented Bacterial Isolates From Sea Cucumbers For Antibacterial Activity Fatin Najihah Binti Muhammad Lutfi Sea cucumber is one of the most commercialized seafood products in Asia, including Malaysia, and its consumption has recently increased with recognition of its health beneficial values. This marine invertebrate is surrounded by microbial communities that may provide defence against pathogens and, in turn, the symbiotic relationship could be a source of bioactive compounds. Many research on marine invertebrate-associated bacteria have been carried out, yet limited studies have documented on bacteria from various body parts of sea cucumbers in Malaysia seawaters, specifically on pigmented bacteria. In this study, eight sea cucumbers were collected from Pangkor Island, Perak, and Manukan Island, Sabah, and the specimens were identified as two H. leucospilota, three H. atra, and one each of H. edulis, H. hilla and B. vitiensis. A total of 115 bacterial samples were isolated from various body parts of the sea cucumbers, surrounding sediments and seawaters, and 58 bacterial strains were successfully identified using 16S rRNA gene amplification, DNA sequencing and phylogenetic analysis. Bacteria from the genera Vibrio and Bacillus are the most affiliated with sea cucumber from Pangkor Island and Manukan Island, respectively. Other bacterial strains belong to the genera Acinetobacteria, Staphylococcus, Pseudomonas, Paracoccus, Microbacterium, Brevundinomas, Exiguobacterium and Stenotrophomonas. Phylogenetic analysis revealed that isolates were assigned to three primary cluster consisting of Proteobacteria, Firmicutes and Actinobacteria phylum. Bacterial isolates that showed pigmentation were selected for UV-Vis spectrophotmetry analysis and antibacterial screening using disk diffusion method. The absorbance spectra of crude methanolic extract of Pseudomonas sp. PMCC1 suggested potential phenazine-based pigments, while those from Staphylococcus sp. PMDP, Microbacterium sp. PMCT1 and Acinetobacteria sp. PMDS1 resembling carotenoid pigments. However, more studies are needed to confirm these pigments. Four pigmented bacterial strains exhibited antibacterial activity against at least one pathogenic bacterium, with Pseudomonas sp. PMCC1 showed inhibition to all five tested bacteria, ranging from 13 to 22 mm. This study suggests that the bacteria isolated from sea cucumbers may have great potential in the discovery of useful bioactive compounds. Therefore, further studies are needed to explore this vast pool of bacterial isolates, especially the pigment-producing bacteria and to determine their biotechnological potentiality. Universiti Sains Islam Malaysia 2022-02 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12451 https://oarep.usim.edu.my/bitstreams/41262d5d-933c-4c00-9c4b-6b04b14ea3a4/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/f22203b7-3a98-4b8a-aff8-9b547d2912ba/download 191b4f595c70b6fcbe9af610e8a99d45 https://oarep.usim.edu.my/bitstreams/be30a50b-ff9f-4588-a82f-af870da9577b/download 6ec4fa15f54521fd567c361627b2e543 https://oarep.usim.edu.my/bitstreams/d9c692ea-c389-4909-848a-8db0393729ec/download 733d39c21ee0aca55cdd51ae0cf69485 https://oarep.usim.edu.my/bitstreams/e670fa59-c8fd-49be-addb-d2f557907870/download 3341140c2fa77f3e0ab0d1bf04c4e95e https://oarep.usim.edu.my/bitstreams/26ffc552-17da-4ab6-b755-b2a2b8599765/download 6cdf0db47ff8bef31c9125e035d51489 https://oarep.usim.edu.my/bitstreams/11253bf8-edff-44b5-83e6-37b93e9eb0ee/download dc6e1a4109bef865ab328800e976c02b https://oarep.usim.edu.my/bitstreams/fc1df9a7-37a2-4409-b172-b039d6af1003/download add417f3d38adeaccf3f1bd9c32b811f https://oarep.usim.edu.my/bitstreams/27d3eb4d-2f46-4fd2-8a20-3885e7d1ca54/download b0bcb13085427d61c416d053b62b4e88 https://oarep.usim.edu.my/bitstreams/eac1cbe5-cd52-4c9a-b48d-7430408cb2d9/download f81152c8bf6ac18f77fac7b70ea9abca https://oarep.usim.edu.my/bitstreams/df4bf7e7-c900-40ae-877c-b0aeda64065e/download cf17b4dbecd2f21eae3595770f4d1ab4 https://oarep.usim.edu.my/bitstreams/4829581c-a939-41ed-8e1f-3b3d94830e52/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/d397e167-c9a0-469b-9d8c-2c9a5dabddd8/download e986aeba9de69ebffa8bcab804588f74 https://oarep.usim.edu.my/bitstreams/08e9eefb-68d0-46dd-a4c1-cdaad6e5c3a5/download 7b32e9538d2108b6eb1be8e1f297bf70 https://oarep.usim.edu.my/bitstreams/5f95904c-278b-4dff-b02f-1b363f6a2efc/download 187e8af48f5aa1e85910c300e57641ac https://oarep.usim.edu.my/bitstreams/6ed05a66-0220-4d80-a043-50af0f3e4a8e/download 5a624afb2da79b6e21c91d61a8e4e7fc https://oarep.usim.edu.my/bitstreams/7e40e015-bd94-46d3-a111-98d06f060e10/download a1e564ebe1ed403e98375a6c0936cf90 https://oarep.usim.edu.my/bitstreams/3fc70c01-c58a-47ec-996a-9fb58549cebe/download f581fc9cbb432095040114289aa92f9a https://oarep.usim.edu.my/bitstreams/0fcd41bb-e455-43ef-ab56-e4b8c058cae6/download 64d4e148391bbbef848449be655a0b05 https://oarep.usim.edu.my/bitstreams/e4c37d2f-bb59-479f-8d1d-413056226e23/download 92644da72a1aa2261d130ae41af4d66c https://oarep.usim.edu.my/bitstreams/eab647c8-b48f-4eea-b88a-b77e55c27b22/download b314fa5fba9db777948d921fce248c31 Sea cucumber, Bacteria, Pigment, 16S rRNA gene, Antibacterial activity |