Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was com...
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| المؤلف الرئيسي: | |
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| التنسيق: | أطروحة |
| اللغة: | English |
| منشور في: |
2010
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf |
| الوسوم: |
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| الملخص: | A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. |
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