Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum

A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was com...

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Main Author:	Shim , Siang yian
Format:	Thesis
Language:	English
Published:	2010
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf
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id	my-usm-ep.42578
record_format	uketd_dc
spelling	my-usm-ep.425782019-04-12T05:26:47Z Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum 2010-10 Shim , Siang yian TP1-1185 Chemical technology A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. 2010-10 Thesis http://eprints.usm.my/42578/ http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Shim , Siang yian Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
description	A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein.
format	Thesis
qualification_level	Master's degree
author	Shim , Siang yian
author_facet	Shim , Siang yian
author_sort	Shim , Siang yian
title	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_short	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_full	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_fullStr	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_full_unstemmed	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_sort	analysis of proteins extracted from the leaves of piper sarmentosum
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuteraan Kimia
publishDate	2010
url	http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf
_version_	1747821085841162240

Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum

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