Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol

Production of fatty acid and glycerol from oils are important especially in oleochemical industries. Nowadays, researchers prefer to use enzyme to conduct hydrolysis in order to reduce energy consumption and minimize thermal degradation of the products. The advantages of the enzyme hydrolysis techni...

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Main Author:	Serri, Noor Aziah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/49044/1/NOOR%20AZIAH%20SERRI_hj.pdf
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id	my-usm-ep.49044
record_format	uketd_dc
spelling	my-usm-ep.490442021-04-28T02:59:25Z Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol 2012-07 Serri, Noor Aziah TP1-1185 Chemical technology Production of fatty acid and glycerol from oils are important especially in oleochemical industries. Nowadays, researchers prefer to use enzyme to conduct hydrolysis in order to reduce energy consumption and minimize thermal degradation of the products. The advantages of the enzyme hydrolysis technique include; the use of bio-route technology that only requires a mild temperature, simple operational procedure and low cost as well as energy consumption. The present investigation focuses on hydrolysis of triglyceride to produce free fatty acids and glycerol from crude palm oil (CPO) using Candida rugosa lipase in batch and enzymatic membrane reactor (EMR). At the same time, the recovery of carotenes and tocopherol was also studied. The optimization in hydrolysis of CPO for batch process was carried out using Design of Experiment that focuses on response surface methodology (RSM) to optimize the hydrolysis reaction. The process variables which were taken into account include; enzyme loading, A (0.30 – 0.80 g), oil loading, B (0.15 – 0.35 g/ml), reaction temperature, C (40oC - 50oC) and pH of buffer solution D, (6.5-7.5). The optimum conditions found for the enzymatic hydrolysis of CPO under investigation are: 0.43 grams of enzyme, 0.15 g/ml of oil with temperature of 45oC and buffer solutions at pH 7.0. The yield predicted for fatty acids produced can reach up to 90.95% and the actual value was found to be 90.67% (5.59 x 10-5 mol hr-1 g-1 enzyme). 2012-07 Thesis http://eprints.usm.my/49044/ http://eprints.usm.my/49044/1/NOOR%20AZIAH%20SERRI_hj.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Serri, Noor Aziah Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
description	Production of fatty acid and glycerol from oils are important especially in oleochemical industries. Nowadays, researchers prefer to use enzyme to conduct hydrolysis in order to reduce energy consumption and minimize thermal degradation of the products. The advantages of the enzyme hydrolysis technique include; the use of bio-route technology that only requires a mild temperature, simple operational procedure and low cost as well as energy consumption. The present investigation focuses on hydrolysis of triglyceride to produce free fatty acids and glycerol from crude palm oil (CPO) using Candida rugosa lipase in batch and enzymatic membrane reactor (EMR). At the same time, the recovery of carotenes and tocopherol was also studied. The optimization in hydrolysis of CPO for batch process was carried out using Design of Experiment that focuses on response surface methodology (RSM) to optimize the hydrolysis reaction. The process variables which were taken into account include; enzyme loading, A (0.30 – 0.80 g), oil loading, B (0.15 – 0.35 g/ml), reaction temperature, C (40oC - 50oC) and pH of buffer solution D, (6.5-7.5). The optimum conditions found for the enzymatic hydrolysis of CPO under investigation are: 0.43 grams of enzyme, 0.15 g/ml of oil with temperature of 45oC and buffer solutions at pH 7.0. The yield predicted for fatty acids produced can reach up to 90.95% and the actual value was found to be 90.67% (5.59 x 10-5 mol hr-1 g-1 enzyme).
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Serri, Noor Aziah
author_facet	Serri, Noor Aziah
author_sort	Serri, Noor Aziah
title	Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
title_short	Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
title_full	Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
title_fullStr	Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
title_full_unstemmed	Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol
title_sort	lipase mediated hydrolysis of crude palm oil in enzymatic membrane reactor and recovery of carotenes and tocopherol
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2012
url	http://eprints.usm.my/49044/1/NOOR%20AZIAH%20SERRI_hj.pdf
_version_	1747821978529562624

Lipase Mediated Hydrolysis Of Crude Palm Oil In Enzymatic Membrane Reactor And Recovery Of Carotenes And Tocopherol

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