Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food

Meat processing is a method of enhancing the flavour or preserving the meat but now it was misused by food manufacturers, whereby the expensive meats in the processed food are substituted with cheaper or inferior meats for their economic gain. Complex composition of processed food today has made the...

Full description

Saved in:

Bibliographic Details
Main Author:	Tan, Lee Lee
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QH426-470 Genetics
Online Access:	http://eprints.usm.my/49708/1/TAN%20LEE%20LEE_hj.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.49708
record_format	uketd_dc
spelling	my-usm-ep.497082021-08-24T13:45:57Z Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food 2018-09 Tan, Lee Lee QH426-470 Genetics Meat processing is a method of enhancing the flavour or preserving the meat but now it was misused by food manufacturers, whereby the expensive meats in the processed food are substituted with cheaper or inferior meats for their economic gain. Complex composition of processed food today has made the species determination a more difficult task. A simple and practical procedure for identification of meat species origin in processed meat products was developed, whereby a SYBR Green-based duplex real-time polymerase chain reactions (qPCR) approach coupled with a rapid DNA extraction method was developed to detect Sus scrofa DNA in processed food. The qPCR targeted LINE-1, a repetitive element sequence exclusively found in pig genome, together with an internal control based on 16S ribosomal RNA gene. The assay was validated for 1) specificity, 2) sensitivity and 3) robustness on processed meat products. Results showed that assay was highly specific when evaluated against a panel of commonly consumed species. The developed assay is capable of capturing the DNA of 0.001% (w/w) adulterated pork meat in just one and a half hour. A total of 121 commercial meat products were tested. No pork adulteration was detected in all the halal-labelled samples. Similar results were obtained using both developed method and kit. The assay would be particularly useful as an alternative for pork control test in food industry 2018-09 Thesis http://eprints.usm.my/49708/ http://eprints.usm.my/49708/1/TAN%20LEE%20LEE_hj.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH426-470 Genetics
spellingShingle	QH426-470 Genetics Tan, Lee Lee Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
description	Meat processing is a method of enhancing the flavour or preserving the meat but now it was misused by food manufacturers, whereby the expensive meats in the processed food are substituted with cheaper or inferior meats for their economic gain. Complex composition of processed food today has made the species determination a more difficult task. A simple and practical procedure for identification of meat species origin in processed meat products was developed, whereby a SYBR Green-based duplex real-time polymerase chain reactions (qPCR) approach coupled with a rapid DNA extraction method was developed to detect Sus scrofa DNA in processed food. The qPCR targeted LINE-1, a repetitive element sequence exclusively found in pig genome, together with an internal control based on 16S ribosomal RNA gene. The assay was validated for 1) specificity, 2) sensitivity and 3) robustness on processed meat products. Results showed that assay was highly specific when evaluated against a panel of commonly consumed species. The developed assay is capable of capturing the DNA of 0.001% (w/w) adulterated pork meat in just one and a half hour. A total of 121 commercial meat products were tested. No pork adulteration was detected in all the halal-labelled samples. Similar results were obtained using both developed method and kit. The assay would be particularly useful as an alternative for pork control test in food industry
format	Thesis
qualification_level	Master's degree
author	Tan, Lee Lee
author_facet	Tan, Lee Lee
author_sort	Tan, Lee Lee
title	Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
title_short	Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
title_full	Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
title_fullStr	Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
title_full_unstemmed	Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
title_sort	development and evaluation of multiplex real-time pcr for porcine dna detection in processed food
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2018
url	http://eprints.usm.my/49708/1/TAN%20LEE%20LEE_hj.pdf
_version_	1747822012124889088

Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food

Similar Items